摘要
目的 建立同时测定舒脑欣滴丸中尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H含量的液相色谱-质谱联用方法（UPLC-Q-TOF）。方法 采用Waters Acquity BEH C18色谱柱（2.1 mm×100 mm，1.7 μm），流动相为乙腈-甲酸-水体系梯度洗脱，流速为0.4 mL·min-1，PDA检测190~400 nm扫描；进样量：2.0 μL。结果 尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H分别在10.31~206.24，3.28~65.52，6.36~127.24，8.24~164.88，10.18~200.52，3.14~62.72 μg·mL-1内与峰面积呈良好线性关系；精密度、重复性和稳定性良好；加样回收率在99%~101%之间。结论 所建立的UPLC-Q-TOF方法简便、准确、快速、重复性好，可用于舒脑欣滴丸中尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H的含量测定，为该药的质量控制提供依据。
Abstract
OBJECTIVE To establish an LC-MS method for simultaneous determination of six compounds (uridine， guanosine， ferulic acid， ligustilide， senkyunolide I and H in Shunaoxin dropping pills. METHODS The analysis was performed on a Waters Acquity BEH C18 column （2.1 mm×100 mm，1.7 μm）with the mixture of acetonitrile-water-formic acid as the mobile phase， and the flow rate was 0.4 mL·min-1； the PDA detection wavelength was 190-400 nm； the column temperature was room temperature （30 ℃）. RESULTS The linear ranges for uridine， guanosine， ferulic acid， ligustilide， senkyunolide I and H were 10.31-206.24，3.28-65.52，6.36-127.24，8.24-164.88，10.18-200.52，and 3.14-62.72 μg·mL-1，respectively. The average recoveries were between 99%-101% (n=6). CONCLUSION This method is simple， accurate and reliable to determine the contents of the six compounds in Shunaoxin dropping pills for the quality control.
关键词
舒脑欣滴丸 /
液相色谱-质谱联用 /
超高压液相色谱 /
串联四级杆飞行时间质谱 /
藁本内酯 /
洋川芎内酯I
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Key words
Shunaoxin dropping pill /
LC-MS /
UPLC /
Q-TOF-MS /
ligustilide /
senkyunolide I
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阎小青，董林毅，齐欣，白钢.
高效液相色谱串联四级杆飞行时间质谱法同时测定舒脑欣滴丸中藁本内酯、洋川芎内酯I等6种成分含量[J]. 中国药学杂志, 2013, 48(3): 216-219 https://doi.org/10.11669/cpj.2013.03.012
YAN Xiao-qing,DONG Lin-yi,QI Xin,BAI Gang.
Simultaneous Determination of Ligustilide，Senkyunolide I and other Six Components in Shunaoxin Dropping Pills by UPLC-Q-TOF-MS[J]. Chinese Pharmaceutical Journal, 2013, 48(3): 216-219 https://doi.org/10.11669/cpj.2013.03.012
中图分类号：
R917
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参考文献
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